Reporter

Part:BBa_K1036004:Design

Designed by: Zhaopeng Cheng, Xiyu Wu   Group: iGEM13_XMU-China   (2013-09-09)


sfgfp with LVA-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

The sfgfp gene with RBS (BBa_B0034) was amplifed from the biobrick pSB1A3-RBS-sfgfp-TT provided by Peking Unversity via PCR. The LVA-tag and terminator(BBa_B0015) were fused with sfgfp via fusion PCR. The DH5α colony carried sfgfp fusion gene was sequenced after TA-cloning.

Source

The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.

References

1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)